When deciding RT-qPCR experiments, there are two different methods. In this lab chat, Application Specialist Maggie Ma explains the differences between 1-step and 2-step RT-qPCR.
Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.
Quantitative PCR (qPCR) technologies have exploded in the last decade. Commonly used for both diagnostics and basic research, new and exciting advances are being made in such areas as biomarker ...