Amplicon Sequencing Protocol for Genome Targeting Protocol by: Lisa Kanizay and Tom Jacobs, Parrott Lab

Dec 3, 2023 · This procedure describes the workflow for constructing pre-indexed amplicon or target-enrichment libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio® HiFi systems.

Hybridization capture and amplicon-based enrichment are two general techniques used in sequencing to enrich for specific genetic regions (Figure 1). Hybridization capture can be either solution based or …

General considerations for producing amplicon libraries provides essential information on producing and submitting amplicon libraries for sequencing to the NSC.

The xGen Amplicon technology brings many benefits, including a single-tube workflow and robust coverage, and is not limited to a discrete number of amplicons in an overlapping amplicon set, like …

Longer amplicon sequencing (> 500 bp) GENEWIZ’s standard amplicon sequencing service is an efective solution for longer amplicons, more coverage, or customized options.

Clean the amplicon pool using Ultra clean 96 PCR Cleanup kit as indicated in the manufacturer’s instructions. After cleaning, this is the final pool 16S rRNA V4 library. Quantify your pooled library …