This online calculator is used to determine and graph the IC50 (half maximal inhibitory concentration) value given a set of experimental data. Data can be from Excel or CSV.

Calculate IC50 values from dose-response data. Fit 4-parameter logistic curves and determine half maximal inhibitory concentration for drug screening.

Note: This calculator uses a 4-parameter logistic (4-PL) regression model with Levenberg-Marquardt optimization. At least 4 data points are recommended.

Very Simple IC50 Tool Kit - calculate, measure, determine IC50 online (Python version). Obtain IC50, EC50, etc, by online curve-fitting and get publication-quality plots! Paste in the X and Y values here.

Calculate IC50 values with 4-parameter logistic curve fitting. Upload your dose-response data and get publication-ready results in seconds.

Concentration 1 (uM): Inhibition Percentage 1 (%):

Input comma-separated values for concentrations and response percentages to calculate the IC50 using linear regression. Obtain immediate results for your bioassay data.

Whether you're studying enzyme kinetics, drug potency, or toxicology, the IC50 Calculator provides a reliable and efficient solution for determining IC50 values.

Master IC50 and EC50 analysis. Learn how to use online Cell Doubling and IC50 Calculators to optimize seeding, normalize data, and determine drug potency accurately.

This guide helps you use IC50/EC50 calculator (dose–response curve fit: 4PL/5PL) in a repeatable way: set a baseline, change one variable at a time, and interpret the output with clear assumptions before …