Video: PCR: Principle, Instrumentation, and Applications
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Video: PCR: Principle, Instrumentation, and Applications
772.4K Views. The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling – cyles of temperature changes at fixed time intervals. Using a thermostable DNA polymerase, PCR can create numerous copies of DNA from DNA building blocks called dNTPs. There are three steps in PCR: denaturation, annealing, and ...
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Rashi Gupta | PhD Scholar on Instagram: "“Polymerase Chain Reaction (PCR) relies on three fundamental steps—denaturation, annealing, and extension🧬—to achieve precise and exponential amplification of a target DNA sequence. During the denaturation phase (94–98°C), the double-stranded DNA unwinds and separates into single strands by breaking hydrogen bonds, preparing the template for amplification. In the annealing step (50–65°C), the temperature is lowered to allow sequence-specific primers to h
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